The Diet-Gut Microbiome Interaction in Type-1 Diabetic and Type-1 Diabetic Obese Pediatric Patients in Qatar

Abstract

The prevalence of non-communicable diseases (NCD) such as diabetes and obesity in Qatar is one of the highest rates among few countries in the world. Many studies have demonstrated microbiome as a potential factor contributing to the pathogenesis of NCD. To our knowledge, none of the previous studies has examined the gut microbiome pattern in metabolically compromised children in Qatar’s population. Type 1 diabetes mellitus (T1DM) is a common NCD among children in Qatar population, and interestingly a group of these children are obese. Hence, the objective of this study is to identify peculiar gut microbiome profiles in T1DM and T1DM-obese children in Qatar’s population. This study involves 70 pediatric subjects aged 6 to 12 years old divided into 4 categories: healthy control (HC), T1DM, T1DM-Obese and Obese. Anthropometric parameters, clinical biomarkers, treatments, and 24-hrs dietary recalls were collected. The microbiome characterization is based on 16S rDNA V3-V4 region using Illumina Miseq platform. QIIME 1.9.0 pipeline, R package, LEfSe and PICRUSt were all used for microbial analysis. Statistical analysis is considered significant when P

Date of Award	2020
Original language	American English
Awarding Institution	HBKU College of Health & Life Sciences