Zfp206 is a transcription factor that controls pluripotency of embryonic stem cells

Zheng Xu Wang; Jacqueline L.L. Kueh; Christina Hui Leng Teh; Michael Rossbach; Linda Lim; Pin Li; Kee Yew Wong; Thomas Lufkin; Paul Robson; Lawrence W. Stanton

doi:10.1634/stemcells.2007-0085

Zfp206 is a transcription factor that controls pluripotency of embryonic stem cells

Zheng Xu Wang, Jacqueline L.L. Kueh, Christina Hui Leng Teh, Michael Rossbach, Linda Lim, Pin Li, Kee Yew Wong, Thomas Lufkin, Paul Robson, Lawrence W. Stanton^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

Zfp206 (ZNF206 in human) encodes a zinc finger- and SCAN domain-containing protein that is highly expressed in pluripotent ESC. Upon differentiation of human and mouse ESC, Zfp206 expression is quickly repressed. Zfp206 was found to be expressed throughout embryogenesis but absent in adult tissues except testis. We have identified a role for Zfp206 in controlling ESC differentiation. ESC engineered to overexpress Zfp206 were found to be resistant to differentiation induced by retinoic acid. In addition, ESC with knocked-down expression of Zfp206 were more sensitive to differentiation by retinoic acid treatment. We found that Zfp206 was able to enhance expression from its own promoter and also activate transcription of the Oct4 and Nanog promoters. Our results show that Zfp206 is an embryonic transcription factor that plays a role in regulating pluripotency of embryonic stem cells.

Original language	English
Pages (from-to)	2173-2182
Number of pages	10
Journal	Stem Cells
Volume	25
Issue number	9
DOIs	https://doi.org/10.1634/stemcells.2007-0085
Publication status	Published - Sept 2007
Externally published	Yes

Keywords

Differentiation
Gene expression
Pluripotency
Stem cells
Transcription factor

Access to Document

10.1634/stemcells.2007-0085

Cite this

@article{3918fd24d9d14f97bfaae43f15e278ba,

title = "Zfp206 is a transcription factor that controls pluripotency of embryonic stem cells",

abstract = "Zfp206 (ZNF206 in human) encodes a zinc finger- and SCAN domain-containing protein that is highly expressed in pluripotent ESC. Upon differentiation of human and mouse ESC, Zfp206 expression is quickly repressed. Zfp206 was found to be expressed throughout embryogenesis but absent in adult tissues except testis. We have identified a role for Zfp206 in controlling ESC differentiation. ESC engineered to overexpress Zfp206 were found to be resistant to differentiation induced by retinoic acid. In addition, ESC with knocked-down expression of Zfp206 were more sensitive to differentiation by retinoic acid treatment. We found that Zfp206 was able to enhance expression from its own promoter and also activate transcription of the Oct4 and Nanog promoters. Our results show that Zfp206 is an embryonic transcription factor that plays a role in regulating pluripotency of embryonic stem cells.",

keywords = "Differentiation, Gene expression, Pluripotency, Stem cells, Transcription factor",

author = "Wang, \{Zheng Xu\} and Kueh, \{Jacqueline L.L.\} and Teh, \{Christina Hui Leng\} and Michael Rossbach and Linda Lim and Pin Li and Wong, \{Kee Yew\} and Thomas Lufkin and Paul Robson and Stanton, \{Lawrence W.\}",

year = "2007",

month = sep,

doi = "10.1634/stemcells.2007-0085",

language = "English",

volume = "25",

pages = "2173--2182",

journal = "Stem Cells",

issn = "1066-5099",

publisher = "Oxford University Press",

number = "9",

}

TY - JOUR

T1 - Zfp206 is a transcription factor that controls pluripotency of embryonic stem cells

AU - Wang, Zheng Xu

AU - Kueh, Jacqueline L.L.

AU - Teh, Christina Hui Leng

AU - Rossbach, Michael

AU - Lim, Linda

AU - Li, Pin

AU - Wong, Kee Yew

AU - Lufkin, Thomas

AU - Robson, Paul

AU - Stanton, Lawrence W.

PY - 2007/9

Y1 - 2007/9

N2 - Zfp206 (ZNF206 in human) encodes a zinc finger- and SCAN domain-containing protein that is highly expressed in pluripotent ESC. Upon differentiation of human and mouse ESC, Zfp206 expression is quickly repressed. Zfp206 was found to be expressed throughout embryogenesis but absent in adult tissues except testis. We have identified a role for Zfp206 in controlling ESC differentiation. ESC engineered to overexpress Zfp206 were found to be resistant to differentiation induced by retinoic acid. In addition, ESC with knocked-down expression of Zfp206 were more sensitive to differentiation by retinoic acid treatment. We found that Zfp206 was able to enhance expression from its own promoter and also activate transcription of the Oct4 and Nanog promoters. Our results show that Zfp206 is an embryonic transcription factor that plays a role in regulating pluripotency of embryonic stem cells.

AB - Zfp206 (ZNF206 in human) encodes a zinc finger- and SCAN domain-containing protein that is highly expressed in pluripotent ESC. Upon differentiation of human and mouse ESC, Zfp206 expression is quickly repressed. Zfp206 was found to be expressed throughout embryogenesis but absent in adult tissues except testis. We have identified a role for Zfp206 in controlling ESC differentiation. ESC engineered to overexpress Zfp206 were found to be resistant to differentiation induced by retinoic acid. In addition, ESC with knocked-down expression of Zfp206 were more sensitive to differentiation by retinoic acid treatment. We found that Zfp206 was able to enhance expression from its own promoter and also activate transcription of the Oct4 and Nanog promoters. Our results show that Zfp206 is an embryonic transcription factor that plays a role in regulating pluripotency of embryonic stem cells.

KW - Differentiation

KW - Gene expression

KW - Pluripotency

KW - Stem cells

KW - Transcription factor

UR - https://www.scopus.com/pages/publications/34748839596

U2 - 10.1634/stemcells.2007-0085

DO - 10.1634/stemcells.2007-0085

M3 - Article

C2 - 17628018

AN - SCOPUS:34748839596

SN - 1066-5099

VL - 25

SP - 2173

EP - 2182

JO - Stem Cells

JF - Stem Cells

IS - 9

ER -

Zfp206 is a transcription factor that controls pluripotency of embryonic stem cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this