Viral Qβ RNA as a high expression vector for mRNA translation in a cell-free system

Vladimir L. Katanaev; Oleg V. Kurnasov; Alexander S. Spirin

doi:10.1016/0014-5793(95)00006-U

Viral Qβ RNA as a high expression vector for mRNA translation in a cell-free system

Vladimir L. Katanaev, Oleg V. Kurnasov, Alexander S. Spirin^*

^*Corresponding author for this work

Institute of Protein Research of the Russian Academy of Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Dihydrofolate reductase (DHFR) mRNA was inserted into Qβ phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Qβ replicase as compared with its synthesis when the original Qβ RNA was used.

Original language	English
Pages (from-to)	89-92
Number of pages	4
Journal	FEBS Letters
Volume	359
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00006-U
Publication status	Published - 6 Feb 1995
Externally published	Yes

Keywords

Cell-free translation
Coat protein gene
Dihydrofolate reductase gene
Qβ phage RNA
Recombinant mRNA

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10.1016/0014-5793(95)00006-U

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title = "Viral Qβ RNA as a high expression vector for mRNA translation in a cell-free system",

abstract = "Dihydrofolate reductase (DHFR) mRNA was inserted into Qβ phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Qβ replicase as compared with its synthesis when the original Qβ RNA was used.",

keywords = "Cell-free translation, Coat protein gene, Dihydrofolate reductase gene, Qβ phage RNA, Recombinant mRNA",

author = "Katanaev, \{Vladimir L.\} and Kurnasov, \{Oleg V.\} and Spirin, \{Alexander S.\}",

year = "1995",

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doi = "10.1016/0014-5793(95)00006-U",

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T1 - Viral Qβ RNA as a high expression vector for mRNA translation in a cell-free system

AU - Katanaev, Vladimir L.

AU - Kurnasov, Oleg V.

AU - Spirin, Alexander S.

PY - 1995/2/6

Y1 - 1995/2/6

N2 - Dihydrofolate reductase (DHFR) mRNA was inserted into Qβ phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Qβ replicase as compared with its synthesis when the original Qβ RNA was used.

AB - Dihydrofolate reductase (DHFR) mRNA was inserted into Qβ phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Qβ replicase as compared with its synthesis when the original Qβ RNA was used.

KW - Cell-free translation

KW - Coat protein gene

KW - Dihydrofolate reductase gene

KW - Qβ phage RNA

KW - Recombinant mRNA

UR - https://www.scopus.com/pages/publications/0028983011

U2 - 10.1016/0014-5793(95)00006-U

DO - 10.1016/0014-5793(95)00006-U

M3 - Article

C2 - 7851538

AN - SCOPUS:0028983011

SN - 0014-5793

VL - 359

SP - 89

EP - 92

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Viral Qβ RNA as a high expression vector for mRNA translation in a cell-free system

Abstract

Keywords

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