The impact of low-frequency and rare variants on lipid levels

Ida Surakka; Momoko Horikoshi; Reedik Mägi; Antti Pekka Sarin; Anubha Mahajan; Vasiliki Lagou; Letizia Marullo; Teresa Ferreira; Benjamin Miraglio; Sanna Timonen; Johannes Kettunen; Matti Pirinen; Juha Karjalainen; Gudmar Thorleifsson; Sara Hägg; Jouke Jan Hottenga; Aaron Isaacs; Claes Ladenvall; Marian Beekman; Tõnu Esko; Janina S. Ried; Christopher P. Nelson; Christina Willenborg; Stefan Gustafsson; Harm Jan Westra; Matthew Blades; Anton J.M. De Craen; Eco J. De Geus; Joris Deelen; Harald Grallert; Anders Hamsten; Aki S. Havulinna; Christian Hengstenberg; Jeanine J. Houwing-Duistermaat; Elina Hyppönen; Lennart C. Karssen; Terho Lehtimäki; Valeriya Lyssenko; Patrik K.E. Magnusson; Evelin Mihailov; Martina Müller-Nurasyid; John Patrick Mpindi; Nancy L. Pedersen; Brenda W.J.H. Penninx; Markus Perola; Tune H. Pers; Annette Peters; Johan Rung; Johannes H. Smit; Valgerdur Steinthorsdottir; Martin D. Tobin; Natalia Tsernikova; Elisabeth M. Van Leeuwen; Jorma S. Viikari; Sara M. Willems; Gonneke Willemsen; Heribert Schunkert; Jeanette Erdmann; Nilesh J. Samani; Jaakko Kaprio; Lars Lind; Christian Gieger; Andres Metspalu; P. Eline Slagboom; Leif Groop; Cornelia M. Van Duijn; Johan G. Eriksson; Antti Jula; Veikko Salomaa; Dorret I. Boomsma; Christine Power; Olli T. Raitakari; Erik Ingelsson; Marjo Riitta Järvelin; Unnur Thorsteinsdottir; Lude Franke; Elina Ikonen; Olli Kallioniemi; Vilja Pietiäinen; Cecilia M. Lindgren; Kari Stefansson; Aarno Palotie; Mark I. McCarthy; Andrew P. Morris; Inga Prokopenko; Samuli Ripatti

doi:10.1038/ng.3300

The impact of low-frequency and rare variants on lipid levels

Ida Surakka
, Momoko Horikoshi
, Reedik Mägi
, Antti Pekka Sarin
, Anubha Mahajan
, Vasiliki Lagou
, Letizia Marullo
, Teresa Ferreira
, Benjamin Miraglio
, Sanna Timonen
, Johannes Kettunen
, Matti Pirinen
, Juha Karjalainen
, Gudmar Thorleifsson
, Sara Hägg
, Jouke Jan Hottenga
, Aaron Isaacs
, Claes Ladenvall
, Marian Beekman
, Tõnu Esko

Janina S. Ried, Christopher P. Nelson, Christina Willenborg, Stefan Gustafsson, Harm Jan Westra, Matthew Blades, Anton J.M. De Craen, Eco J. De Geus, Joris Deelen, Harald Grallert, Anders Hamsten, Aki S. Havulinna, Christian Hengstenberg, Jeanine J. Houwing-Duistermaat, Elina Hyppönen, Lennart C. Karssen, Terho Lehtimäki, Valeriya Lyssenko, Patrik K.E. Magnusson, Evelin Mihailov, Martina Müller-Nurasyid, John Patrick Mpindi, Nancy L. Pedersen, Brenda W.J.H. Penninx, Markus Perola, Tune H. Pers, Annette Peters, Johan Rung, Johannes H. Smit, Valgerdur Steinthorsdottir, Martin D. Tobin, Natalia Tsernikova, Elisabeth M. Van Leeuwen, Jorma S. Viikari, Sara M. Willems, Gonneke Willemsen, Heribert Schunkert, Jeanette Erdmann, Nilesh J. Samani, Jaakko Kaprio, Lars Lind, Christian Gieger, Andres Metspalu, P. Eline Slagboom, Leif Groop, Cornelia M. Van Duijn, Johan G. Eriksson, Antti Jula, Veikko Salomaa, Dorret I. Boomsma, Christine Power, Olli T. Raitakari, Erik Ingelsson, Marjo Riitta Järvelin, Unnur Thorsteinsdottir, Lude Franke, Elina Ikonen, Olli Kallioniemi, Vilja Pietiäinen, Cecilia M. Lindgren, Kari Stefansson, Aarno Palotie, Mark I. McCarthy, Andrew P. Morris, Inga Prokopenko, Samuli Ripatti^*

^*Corresponding author for this work

University of Helsinki
National Institute for Health and Welfare
University of Oxford
University of Tartu
University of Ferrara
University of Groningen
deCODE Genetics
Karolinska Institutet
Uppsala University
University of Amsterdam
Erasmus University Rotterdam
Centre for Medical Systems Biology
Lund University
Leiden University
Netherlands Consortium for Healthy Ageing
Boston Children's Hospital
Broad Institute
Harvard University
Helmholtz Zentrum München - German Research Center for Environmental Health
University of Leicester
University Hospitals of Leicester NHS Trust
University of Lübeck
German Centre for Cardiovascular Research
German Center for Diabetes Research
Atherosclerosis Research Unit
Technical University of Munich
University College London
South Australian Health And Medical Research Institute
Adelaide University
Fimlab Laboratories
Novo Nordisk Foundation
Ludwig Maximilian University of Munich
VU University Medical Center
Wellcome Trust
University of Turku
University of Tartu
Folkhalsan
Helsinki University Hospital
Imperial College London
University of Oulu
University of Iceland
Minerva Foundation Institute for Medical Research Helsinki
Massachusetts General Hospital
Oxford University Hospitals NHS Foundation Trust
University of Liverpool
Hammersmith Hospital

Research output: Contribution to journal › Article › peer-review

268 Citations (Scopus)

Abstract

Using a genome-wide screen of 9.6 million genetic variants achieved through 1000 Genomes Project imputation in 62,166 samples, we identify association to lipid traits in 93 loci, including 79 previously identified loci with new lead SNPs and 10 new loci, 15 loci with a low-frequency lead SNP and 10 loci with a missense lead SNP, and 2 loci with an accumulation of rare variants. In six loci, SNPs with established function in lipid genetics (CELSR2, GCKR, LIPC and APOE) or candidate missense mutations with predicted damaging function (CD300LG and TM6SF2) explained the locus associations. The low-frequency variants increased the proportion of variance explained, particularly for low-density lipoprotein cholesterol and total cholesterol. Altogether, our results highlight the impact of low-frequency variants in complex traits and show that imputation offers a cost-effective alternative to resequencing.

Original language	English
Pages (from-to)	589-597
Number of pages	9
Journal	Nature Genetics
Volume	47
Issue number	6
DOIs	https://doi.org/10.1038/ng.3300
Publication status	Published - 11 May 2015
Externally published	Yes

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Surakka, I., Horikoshi, M., Mägi, R., Sarin, A. P., Mahajan, A., Lagou, V., Marullo, L., Ferreira, T., Miraglio, B., Timonen, S., Kettunen, J., Pirinen, M., Karjalainen, J., Thorleifsson, G., Hägg, S., Hottenga, J. J., Isaacs, A., Ladenvall, C., Beekman, M., ... Ripatti, S. (2015). The impact of low-frequency and rare variants on lipid levels. Nature Genetics, 47(6), 589-597. https://doi.org/10.1038/ng.3300

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title = "The impact of low-frequency and rare variants on lipid levels",

abstract = "Using a genome-wide screen of 9.6 million genetic variants achieved through 1000 Genomes Project imputation in 62,166 samples, we identify association to lipid traits in 93 loci, including 79 previously identified loci with new lead SNPs and 10 new loci, 15 loci with a low-frequency lead SNP and 10 loci with a missense lead SNP, and 2 loci with an accumulation of rare variants. In six loci, SNPs with established function in lipid genetics (CELSR2, GCKR, LIPC and APOE) or candidate missense mutations with predicted damaging function (CD300LG and TM6SF2) explained the locus associations. The low-frequency variants increased the proportion of variance explained, particularly for low-density lipoprotein cholesterol and total cholesterol. Altogether, our results highlight the impact of low-frequency variants in complex traits and show that imputation offers a cost-effective alternative to resequencing.",

author = "Ida Surakka and Momoko Horikoshi and Reedik M{\"a}gi and Sarin, \{Antti Pekka\} and Anubha Mahajan and Vasiliki Lagou and Letizia Marullo and Teresa Ferreira and Benjamin Miraglio and Sanna Timonen and Johannes Kettunen and Matti Pirinen and Juha Karjalainen and Gudmar Thorleifsson and Sara H{\"a}gg and Hottenga, \{Jouke Jan\} and Aaron Isaacs and Claes Ladenvall and Marian Beekman and T{\~o}nu Esko and Ried, \{Janina S.\} and Nelson, \{Christopher P.\} and Christina Willenborg and Stefan Gustafsson and Westra, \{Harm Jan\} and Matthew Blades and \{De Craen\}, \{Anton J.M.\} and \{De Geus\}, \{Eco J.\} and Joris Deelen and Harald Grallert and Anders Hamsten and Havulinna, \{Aki S.\} and Christian Hengstenberg and Houwing-Duistermaat, \{Jeanine J.\} and Elina Hypp{\"o}nen and Karssen, \{Lennart C.\} and Terho Lehtim{\"a}ki and Valeriya Lyssenko and Magnusson, \{Patrik K.E.\} and Evelin Mihailov and Martina M{\"u}ller-Nurasyid and Mpindi, \{John Patrick\} and Pedersen, \{Nancy L.\} and Penninx, \{Brenda W.J.H.\} and Markus Perola and Pers, \{Tune H.\} and Annette Peters and Johan Rung and Smit, \{Johannes H.\} and Valgerdur Steinthorsdottir and Tobin, \{Martin D.\} and Natalia Tsernikova and \{Van Leeuwen\}, \{Elisabeth M.\} and Viikari, \{Jorma S.\} and Willems, \{Sara M.\} and Gonneke Willemsen and Heribert Schunkert and Jeanette Erdmann and Samani, \{Nilesh J.\} and Jaakko Kaprio and Lars Lind and Christian Gieger and Andres Metspalu and \{Eline Slagboom\}, P. and Leif Groop and \{Van Duijn\}, \{Cornelia M.\} and Eriksson, \{Johan G.\} and Antti Jula and Veikko Salomaa and Boomsma, \{Dorret I.\} and Christine Power and Raitakari, \{Olli T.\} and Erik Ingelsson and J{\"a}rvelin, \{Marjo Riitta\} and Unnur Thorsteinsdottir and Lude Franke and Elina Ikonen and Olli Kallioniemi and Vilja Pieti{\"a}inen and Lindgren, \{Cecilia M.\} and Kari Stefansson and Aarno Palotie and McCarthy, \{Mark I.\} and Morris, \{Andrew P.\} and Inga Prokopenko and Samuli Ripatti",

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Surakka, I, Horikoshi, M, Mägi, R, Sarin, AP, Mahajan, A, Lagou, V, Marullo, L, Ferreira, T, Miraglio, B, Timonen, S, Kettunen, J, Pirinen, M, Karjalainen, J, Thorleifsson, G, Hägg, S, Hottenga, JJ, Isaacs, A, Ladenvall, C, Beekman, M, Esko, T, Ried, JS, Nelson, CP, Willenborg, C, Gustafsson, S, Westra, HJ, Blades, M, De Craen, AJM, De Geus, EJ, Deelen, J, Grallert, H, Hamsten, A, Havulinna, AS, Hengstenberg, C, Houwing-Duistermaat, JJ, Hyppönen, E, Karssen, LC, Lehtimäki, T, Lyssenko, V, Magnusson, PKE, Mihailov, E, Müller-Nurasyid, M, Mpindi, JP, Pedersen, NL, Penninx, BWJH, Perola, M, Pers, TH, Peters, A, Rung, J, Smit, JH, Steinthorsdottir, V, Tobin, MD, Tsernikova, N, Van Leeuwen, EM, Viikari, JS, Willems, SM, Willemsen, G, Schunkert, H, Erdmann, J, Samani, NJ, Kaprio, J, Lind, L, Gieger, C, Metspalu, A, Eline Slagboom, P, Groop, L, Van Duijn, CM, Eriksson, JG, Jula, A, Salomaa, V, Boomsma, DI, Power, C, Raitakari, OT, Ingelsson, E, Järvelin, MR, Thorsteinsdottir, U, Franke, L, Ikonen, E, Kallioniemi, O, Pietiäinen, V, Lindgren, CM, Stefansson, K, Palotie, A, McCarthy, MI, Morris, AP, Prokopenko, I & Ripatti, S 2015, 'The impact of low-frequency and rare variants on lipid levels', Nature Genetics, vol. 47, no. 6, pp. 589-597. https://doi.org/10.1038/ng.3300

TY - JOUR

T1 - The impact of low-frequency and rare variants on lipid levels

AU - Surakka, Ida

AU - Horikoshi, Momoko

AU - Mägi, Reedik

AU - Sarin, Antti Pekka

AU - Mahajan, Anubha

AU - Lagou, Vasiliki

AU - Marullo, Letizia

AU - Ferreira, Teresa

AU - Miraglio, Benjamin

AU - Timonen, Sanna

AU - Kettunen, Johannes

AU - Pirinen, Matti

AU - Karjalainen, Juha

AU - Thorleifsson, Gudmar

AU - Hägg, Sara

AU - Hottenga, Jouke Jan

AU - Isaacs, Aaron

AU - Ladenvall, Claes

AU - Beekman, Marian

AU - Esko, Tõnu

AU - Ried, Janina S.

AU - Nelson, Christopher P.

AU - Willenborg, Christina

AU - Gustafsson, Stefan

AU - Westra, Harm Jan

AU - Blades, Matthew

AU - De Craen, Anton J.M.

AU - De Geus, Eco J.

AU - Deelen, Joris

AU - Grallert, Harald

AU - Hamsten, Anders

AU - Havulinna, Aki S.

AU - Hengstenberg, Christian

AU - Houwing-Duistermaat, Jeanine J.

AU - Hyppönen, Elina

AU - Karssen, Lennart C.

AU - Lehtimäki, Terho

AU - Lyssenko, Valeriya

AU - Magnusson, Patrik K.E.

AU - Mihailov, Evelin

AU - Müller-Nurasyid, Martina

AU - Mpindi, John Patrick

AU - Pedersen, Nancy L.

AU - Penninx, Brenda W.J.H.

AU - Perola, Markus

AU - Pers, Tune H.

AU - Peters, Annette

AU - Rung, Johan

AU - Smit, Johannes H.

AU - Steinthorsdottir, Valgerdur

AU - Tobin, Martin D.

AU - Tsernikova, Natalia

AU - Van Leeuwen, Elisabeth M.

AU - Viikari, Jorma S.

AU - Willems, Sara M.

AU - Willemsen, Gonneke

AU - Schunkert, Heribert

AU - Erdmann, Jeanette

AU - Samani, Nilesh J.

AU - Kaprio, Jaakko

AU - Lind, Lars

AU - Gieger, Christian

AU - Metspalu, Andres

AU - Eline Slagboom, P.

AU - Groop, Leif

AU - Van Duijn, Cornelia M.

AU - Eriksson, Johan G.

AU - Jula, Antti

AU - Salomaa, Veikko

AU - Boomsma, Dorret I.

AU - Power, Christine

AU - Raitakari, Olli T.

AU - Ingelsson, Erik

AU - Järvelin, Marjo Riitta

AU - Thorsteinsdottir, Unnur

AU - Franke, Lude

AU - Ikonen, Elina

AU - Kallioniemi, Olli

AU - Pietiäinen, Vilja

AU - Lindgren, Cecilia M.

AU - Stefansson, Kari

AU - Palotie, Aarno

AU - McCarthy, Mark I.

AU - Morris, Andrew P.

AU - Prokopenko, Inga

AU - Ripatti, Samuli

PY - 2015/5/11

Y1 - 2015/5/11

N2 - Using a genome-wide screen of 9.6 million genetic variants achieved through 1000 Genomes Project imputation in 62,166 samples, we identify association to lipid traits in 93 loci, including 79 previously identified loci with new lead SNPs and 10 new loci, 15 loci with a low-frequency lead SNP and 10 loci with a missense lead SNP, and 2 loci with an accumulation of rare variants. In six loci, SNPs with established function in lipid genetics (CELSR2, GCKR, LIPC and APOE) or candidate missense mutations with predicted damaging function (CD300LG and TM6SF2) explained the locus associations. The low-frequency variants increased the proportion of variance explained, particularly for low-density lipoprotein cholesterol and total cholesterol. Altogether, our results highlight the impact of low-frequency variants in complex traits and show that imputation offers a cost-effective alternative to resequencing.

AB - Using a genome-wide screen of 9.6 million genetic variants achieved through 1000 Genomes Project imputation in 62,166 samples, we identify association to lipid traits in 93 loci, including 79 previously identified loci with new lead SNPs and 10 new loci, 15 loci with a low-frequency lead SNP and 10 loci with a missense lead SNP, and 2 loci with an accumulation of rare variants. In six loci, SNPs with established function in lipid genetics (CELSR2, GCKR, LIPC and APOE) or candidate missense mutations with predicted damaging function (CD300LG and TM6SF2) explained the locus associations. The low-frequency variants increased the proportion of variance explained, particularly for low-density lipoprotein cholesterol and total cholesterol. Altogether, our results highlight the impact of low-frequency variants in complex traits and show that imputation offers a cost-effective alternative to resequencing.

UR - https://www.scopus.com/pages/publications/84930090376

U2 - 10.1038/ng.3300

DO - 10.1038/ng.3300

M3 - Article

C2 - 25961943

AN - SCOPUS:84930090376

SN - 1061-4036

VL - 47

SP - 589

EP - 597

JO - Nature Genetics

JF - Nature Genetics

IS - 6

ER -

The impact of low-frequency and rare variants on lipid levels

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