Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb; Nadja Kampschulte; Gerhard Hagn; Kathrin Plitzko; Sven W. Meckelmann; Soumita Ghosh; Robin Joshi; Julia Kuligowski; Dajana Vuckovic; Marina T. Botana; Ángel Sánchez-Illana; Fereshteh Zandkarimi; Aditi Das; Jun Yang; Louis Schmidt; Antonio Checa; Helen M. Roche; Aaron M. Armando; Matthew L. Edin; Fred B. Lih; Juan J. Aristizabal-Henao; Sayuri Miyamoto; Francesca Giuffrida; Arieh Moussaieff; Rosário Domingues; Michael Rothe; Christine Hinz; Ujjalkumar Subhash Das; Katharina M. Rund; Ameer Y. Taha; Robert K. Hofstetter; Markus Werner; Oliver Werz; Astrid S. Kahnt; Justine Bertrand-Michel; Pauline Le Faouder; Robert Gurke; Dominique Thomas; Federico Torta; Ivana Milic; Irundika H.K. Dias; Corinne M. Spickett; Denise Biagini; Tommaso Lomonaco; Helena Idborg; Jun Yan Liu; Maria Fedorova; David A. Ford; Anne Barden; Trevor A. Mori; Paul D. Kennedy; Kirk Maxey; Julijana Ivanisevic; Hector Gallart-Ayala; Cécile Gladine; Markus Wenk; Jean Marie Galano; Thierry Durand; Ken D. Stark; Coral Barbas; Ulrike Garscha; Stacy L. Gelhaus; Uta Ceglarek; Nicolas Flamand; Julian L. Griffin; Robert Ahrends; Makoto Arita; Darryl C. Zeldin; Francisco J. Schopfer; Oswald Quehenberger; Randall Julian; Anna Nicolaou; Ian A. Blair; Michael P. Murphy; Bruce D. Hammock; Bruce Freeman; Gerhard Liebisch; Charles N. Serhan; Harald C. Köfeler; Per Johan Jakobsson; Dieter Steinhilber; Michael H. Gelb; Michal Holčapek; Ruth Andrew; Martin Giera; Garret A. FitzGerald; Robert C. Murphy; John W. Newman; Edward A. Dennis; Kim Ekroos; Ginger L. Milne; Miguel A. Gijón; Hubert W. Vesper; Craig E. Wheelock; Valerie B. O'Donnell

doi:10.1126/scisignal.adw1245

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

Nils Helge Schebb^*
, Nadja Kampschulte
, Gerhard Hagn
, Kathrin Plitzko
, Sven W. Meckelmann
, Soumita Ghosh
, Robin Joshi
, Julia Kuligowski
, Dajana Vuckovic
, Marina T. Botana
, Ángel Sánchez-Illana
, Fereshteh Zandkarimi
, Aditi Das
, Jun Yang
, Louis Schmidt
, Antonio Checa
, Helen M. Roche
, Aaron M. Armando
, Matthew L. Edin
, Fred B. Lih

Juan J. Aristizabal-Henao, Sayuri Miyamoto, Francesca Giuffrida, Arieh Moussaieff, Rosário Domingues, Michael Rothe, Christine Hinz, Ujjalkumar Subhash Das, Katharina M. Rund, Ameer Y. Taha, Robert K. Hofstetter, Markus Werner, Oliver Werz, Astrid S. Kahnt, Justine Bertrand-Michel, Pauline Le Faouder, Robert Gurke, Dominique Thomas, Federico Torta, Ivana Milic, Irundika H.K. Dias, Corinne M. Spickett, Denise Biagini, Tommaso Lomonaco, Helena Idborg, Jun Yan Liu, Maria Fedorova, David A. Ford, Anne Barden, Trevor A. Mori, Paul D. Kennedy, Kirk Maxey, Julijana Ivanisevic, Hector Gallart-Ayala, Cécile Gladine, Markus Wenk, Jean Marie Galano, Thierry Durand, Ken D. Stark, Coral Barbas, Ulrike Garscha, Stacy L. Gelhaus, Uta Ceglarek, Nicolas Flamand, Julian L. Griffin, Robert Ahrends, Makoto Arita, Darryl C. Zeldin, Francisco J. Schopfer, Oswald Quehenberger, Randall Julian, Anna Nicolaou, Ian A. Blair, Michael P. Murphy, Bruce D. Hammock, Bruce Freeman, Gerhard Liebisch, Charles N. Serhan, Harald C. Köfeler, Per Johan Jakobsson, Dieter Steinhilber, Michael H. Gelb, Michal Holčapek, Ruth Andrew, Martin Giera, Garret A. FitzGerald, Robert C. Murphy, John W. Newman, Edward A. Dennis, Kim Ekroos, Ginger L. Milne, Miguel A. Gijón, Hubert W. Vesper, Craig E. Wheelock, Valerie B. O'Donnell

^*Corresponding author for this work

HBKU College of Health & Life Sciences

School of Mathematics and Natural Sciences
University of Wuppertal
Unit of Integrative Metabolomics
Karolinska Institutet
Applied Analytical Chemistry
University of Duisburg-Essen
University of Pennsylvania
Neonatal Research Group
Instituto de Investigación Sanitaria La Fe
Department of Chemistry and Biochemistry
Concordia University
School of Biological Sciences
Victoria University of Wellington
Department of Analytical Chemistry
University of Valencia
Mass Spectrometry Core Facility
Columbia University
Georgia Institute of Technology
Department of Entomology
University of California at Davis
Department of Pharmaceutical and Medicinal Chemistry
University of Greifswald
Nutrigenomics Research Group
University College Dublin
Institute for Global Food Security
Queen's University Belfast
University of California at San Diego
Division of Intramural Research
National Institutes of Health
BPG Bio
Department of Biochemistry
Universidade de São Paulo
Nestle
Hebrew University of Jerusalem
CESAM- Centre for Environmental and Marine Studies
University of Aveiro
Lipidomix GmbH
Shimadzu
University of California
Department of Pharmaceutical/Medicinal Chemistry
Friedrich Schiller University Jena
Institute of Pharmaceutical Chemistry
Goethe University Frankfurt
INSERM UMR 1048
Faculty of Medicine
Fraunhofer Institute for Translational Medicine and Pharmacology ITMP
Fraunhofer Cluster of Excellence of Immune Mediate Diseases CIMD
Signature Research Program in Cardiovascular and Metabolic Disorders
Duke-NUS Medical School
Department of Biochemistry
National University of Singapore
School of Biosciences
Aston University
College of Health and Life Sciences
Department of Chemistry and Industrial Chemistry
University of Pisa
Atherosclerosis Research Unit
Center for Novel Target & Therapeutic Intervention
Chongqing Medical University
Center of Membrane Biochemistry and Lipid Research
Technische Universität Dresden
Saint Louis University
University of Western Australia
Cayman Chemical
Metabolomics and Lipidomics Platform
University of Lausanne
Unité de Nutrition Humaine (UNH)
Institut des Biomolécules Max Mousseron
Department of Kinesiology and Health Sciences
University of Waterloo
Centre for Metabolomics and Bioanalysis (CEMBIO)
CEU Universities
Health Sciences Mass Spectrometry Core
University of Pittsburgh
Institute of Laboratory Medicine
Leipzig University
Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec
Université Laval
Rowett Institute
University of Aberdeen
Department of Analytical Chemistry
University of Vienna
RIKEN
Indigo BioAutomation
Division of Pharmacy and Optometry
University of Manchester
Department of Systems Pharmacology and Translational Therapeutics
University of Cambridge
Institute of Clinical Chemistry and Laboratory Medicine
University of Regensburg
Center for Experimental Therapeutics and Reperfusion Injury
Harvard University
Core Facility for Mass Spectrometry
Medical University of Graz
Department of Chemistry
University of Washington
University of Pardubice
University of Edinburgh
Leiden University
University of Colorado Anschutz Medical Campus
Lipidomics Consulting Ltd.
Division of Clinical Pharmacology
Vanderbilt University
Centers for Disease Control and Prevention
Department of Respiratory Medicine and Allergy
Cardiff University

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

Original language	English
Pages (from-to)	eadw1245
Journal	Science Signaling
Volume	18
Issue number	887
DOIs	https://doi.org/10.1126/scisignal.adw1245
Publication status	Published - 20 May 2025

Keywords

Prostaglandin

Access to Document

10.1126/scisignal.adw1245

Cite this

Schebb, N. H., Kampschulte, N., Hagn, G., Plitzko, K., Meckelmann, S. W., Ghosh, S., Joshi, R., Kuligowski, J., Vuckovic, D., Botana, M. T., Sánchez-Illana, Á., Zandkarimi, F., Das, A., Yang, J., Schmidt, L., Checa, A., Roche, H. M., Armando, A. M., Edin, M. L., ... O'Donnell, V. B. (2025). Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry. Science Signaling, 18(887), eadw1245. https://doi.org/10.1126/scisignal.adw1245

@article{7404a7630d59446d963030d6c06f5644,

title = "Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry",

abstract = "Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.",

keywords = "Prostaglandin",

author = "Schebb, \{Nils Helge\} and Nadja Kampschulte and Gerhard Hagn and Kathrin Plitzko and Meckelmann, \{Sven W.\} and Soumita Ghosh and Robin Joshi and Julia Kuligowski and Dajana Vuckovic and Botana, \{Marina T.\} and {\'A}ngel S{\'a}nchez-Illana and Fereshteh Zandkarimi and Aditi Das and Jun Yang and Louis Schmidt and Antonio Checa and Roche, \{Helen M.\} and Armando, \{Aaron M.\} and Edin, \{Matthew L.\} and Lih, \{Fred B.\} and Aristizabal-Henao, \{Juan J.\} and Sayuri Miyamoto and Francesca Giuffrida and Arieh Moussaieff and Ros{\'a}rio Domingues and Michael Rothe and Christine Hinz and Das, \{Ujjalkumar Subhash\} and Rund, \{Katharina M.\} and Taha, \{Ameer Y.\} and Hofstetter, \{Robert K.\} and Markus Werner and Oliver Werz and Kahnt, \{Astrid S.\} and Justine Bertrand-Michel and \{Le Faouder\}, Pauline and Robert Gurke and Dominique Thomas and Federico Torta and Ivana Milic and Dias, \{Irundika H.K.\} and Spickett, \{Corinne M.\} and Denise Biagini and Tommaso Lomonaco and Helena Idborg and Liu, \{Jun Yan\} and Maria Fedorova and Ford, \{David A.\} and Anne Barden and Mori, \{Trevor A.\} and Kennedy, \{Paul D.\} and Kirk Maxey and Julijana Ivanisevic and Hector Gallart-Ayala and C{\'e}cile Gladine and Markus Wenk and Galano, \{Jean Marie\} and Thierry Durand and Stark, \{Ken D.\} and Coral Barbas and Ulrike Garscha and Gelhaus, \{Stacy L.\} and Uta Ceglarek and Nicolas Flamand and Griffin, \{Julian L.\} and Robert Ahrends and Makoto Arita and Zeldin, \{Darryl C.\} and Schopfer, \{Francisco J.\} and Oswald Quehenberger and Randall Julian and Anna Nicolaou and Blair, \{Ian A.\} and Murphy, \{Michael P.\} and Hammock, \{Bruce D.\} and Bruce Freeman and Gerhard Liebisch and Serhan, \{Charles N.\} and K{\"o}feler, \{Harald C.\} and Jakobsson, \{Per Johan\} and Dieter Steinhilber and Gelb, \{Michael H.\} and Michal Hol{\v c}apek and Ruth Andrew and Martin Giera and FitzGerald, \{Garret A.\} and Murphy, \{Robert C.\} and Newman, \{John W.\} and Dennis, \{Edward A.\} and Kim Ekroos and Milne, \{Ginger L.\} and Gij{\'o}n, \{Miguel A.\} and Vesper, \{Hubert W.\} and Wheelock, \{Craig E.\} and O'Donnell, \{Valerie B.\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2025 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.",

year = "2025",

month = may,

day = "20",

doi = "10.1126/scisignal.adw1245",

language = "English",

volume = "18",

pages = "eadw1245",

journal = "Science Signaling",

issn = "1945-0877",

publisher = "American Association for the Advancement of Science",

number = "887",

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Schebb, NH, Kampschulte, N, Hagn, G, Plitzko, K, Meckelmann, SW, Ghosh, S, Joshi, R, Kuligowski, J, Vuckovic, D, Botana, MT, Sánchez-Illana, Á, Zandkarimi, F, Das, A, Yang, J, Schmidt, L, Checa, A, Roche, HM, Armando, AM, Edin, ML, Lih, FB, Aristizabal-Henao, JJ, Miyamoto, S, Giuffrida, F, Moussaieff, A, Domingues, R, Rothe, M, Hinz, C, Das, US, Rund, KM, Taha, AY, Hofstetter, RK, Werner, M, Werz, O, Kahnt, AS, Bertrand-Michel, J, Le Faouder, P, Gurke, R, Thomas, D, Torta, F, Milic, I, Dias, IHK, Spickett, CM, Biagini, D, Lomonaco, T, Idborg, H, Liu, JY, Fedorova, M, Ford, DA, Barden, A, Mori, TA, Kennedy, PD, Maxey, K, Ivanisevic, J, Gallart-Ayala, H, Gladine, C, Wenk, M, Galano, JM, Durand, T, Stark, KD, Barbas, C, Garscha, U, Gelhaus, SL, Ceglarek, U, Flamand, N, Griffin, JL, Ahrends, R, Arita, M, Zeldin, DC, Schopfer, FJ, Quehenberger, O, Julian, R, Nicolaou, A, Blair, IA, Murphy, MP, Hammock, BD, Freeman, B, Liebisch, G, Serhan, CN, Köfeler, HC, Jakobsson, PJ, Steinhilber, D, Gelb, MH, Holčapek, M, Andrew, R, Giera, M, FitzGerald, GA, Murphy, RC, Newman, JW, Dennis, EA, Ekroos, K, Milne, GL, Gijón, MA, Vesper, HW, Wheelock, CE & O'Donnell, VB 2025, 'Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry', Science Signaling, vol. 18, no. 887, pp. eadw1245. https://doi.org/10.1126/scisignal.adw1245

TY - JOUR

T1 - Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

AU - Schebb, Nils Helge

AU - Kampschulte, Nadja

AU - Hagn, Gerhard

AU - Plitzko, Kathrin

AU - Meckelmann, Sven W.

AU - Ghosh, Soumita

AU - Joshi, Robin

AU - Kuligowski, Julia

AU - Vuckovic, Dajana

AU - Botana, Marina T.

AU - Sánchez-Illana, Ángel

AU - Zandkarimi, Fereshteh

AU - Das, Aditi

AU - Yang, Jun

AU - Schmidt, Louis

AU - Checa, Antonio

AU - Roche, Helen M.

AU - Armando, Aaron M.

AU - Edin, Matthew L.

AU - Lih, Fred B.

AU - Aristizabal-Henao, Juan J.

AU - Miyamoto, Sayuri

AU - Giuffrida, Francesca

AU - Moussaieff, Arieh

AU - Domingues, Rosário

AU - Rothe, Michael

AU - Hinz, Christine

AU - Das, Ujjalkumar Subhash

AU - Rund, Katharina M.

AU - Taha, Ameer Y.

AU - Hofstetter, Robert K.

AU - Werner, Markus

AU - Werz, Oliver

AU - Kahnt, Astrid S.

AU - Bertrand-Michel, Justine

AU - Le Faouder, Pauline

AU - Gurke, Robert

AU - Thomas, Dominique

AU - Torta, Federico

AU - Milic, Ivana

AU - Dias, Irundika H.K.

AU - Spickett, Corinne M.

AU - Biagini, Denise

AU - Lomonaco, Tommaso

AU - Idborg, Helena

AU - Liu, Jun Yan

AU - Fedorova, Maria

AU - Ford, David A.

AU - Barden, Anne

AU - Mori, Trevor A.

AU - Kennedy, Paul D.

AU - Maxey, Kirk

AU - Ivanisevic, Julijana

AU - Gallart-Ayala, Hector

AU - Gladine, Cécile

AU - Wenk, Markus

AU - Galano, Jean Marie

AU - Durand, Thierry

AU - Stark, Ken D.

AU - Barbas, Coral

AU - Garscha, Ulrike

AU - Gelhaus, Stacy L.

AU - Ceglarek, Uta

AU - Flamand, Nicolas

AU - Griffin, Julian L.

AU - Ahrends, Robert

AU - Arita, Makoto

AU - Zeldin, Darryl C.

AU - Schopfer, Francisco J.

AU - Quehenberger, Oswald

AU - Julian, Randall

AU - Nicolaou, Anna

AU - Blair, Ian A.

AU - Murphy, Michael P.

AU - Hammock, Bruce D.

AU - Freeman, Bruce

AU - Liebisch, Gerhard

AU - Serhan, Charles N.

AU - Köfeler, Harald C.

AU - Jakobsson, Per Johan

AU - Steinhilber, Dieter

AU - Gelb, Michael H.

AU - Holčapek, Michal

AU - Andrew, Ruth

AU - Giera, Martin

AU - FitzGerald, Garret A.

AU - Murphy, Robert C.

AU - Newman, John W.

AU - Dennis, Edward A.

AU - Ekroos, Kim

AU - Milne, Ginger L.

AU - Gijón, Miguel A.

AU - Vesper, Hubert W.

AU - Wheelock, Craig E.

AU - O'Donnell, Valerie B.

PY - 2025/5/20

Y1 - 2025/5/20

N2 - Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

AB - Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

KW - Prostaglandin

UR - https://www.scopus.com/pages/publications/105006531246

U2 - 10.1126/scisignal.adw1245

DO - 10.1126/scisignal.adw1245

M3 - Article

C2 - 40392938

AN - SCOPUS:105006531246

SN - 1945-0877

VL - 18

SP - eadw1245

JO - Science Signaling

JF - Science Signaling

IS - 887

ER -

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry

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Keywords

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