Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain: AC voltammetry, impedance spectroscopy and surface plasmon resonance studies

Chikka N. Tharamani; Khaled A. Mahmoud; Ganga Ramu Vasanthakumar; Heinz Bernard Kraatz

doi:10.1016/j.snb.2008.11.050

Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain: AC voltammetry, impedance spectroscopy and surface plasmon resonance studies

Chikka N. Tharamani^*
, Khaled A. Mahmoud
, Ganga Ramu Vasanthakumar
, Heinz Bernard Kraatz

^*Corresponding author for this work

University of Saskatchewan
Max Planck Institute of Molecular Physiology

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The interaction of papain with peptide immobilized on the Au surface altered the interfacial electron transfer resistant, charge transfer resistance (R_CT), by preventing the redox species approaching the electrode was characterized by electrochemical impedance spectroscopy (EIS), AC voltammetry, cyclic voltammetry and surface plasmon resonance techniques (SPR). From cyclic voltammetry a potential shift of 25-30 mV was observed after the addition of papain, with decrease in current and the impedance results showed that R_CT increased with increasing concentration of papain indicating the interaction of papain with the immobilized film. This change in kinetics was also observed in SPR. The interaction was further confirmed by reflection-absorption infrared spectroscopy (RAIR).

Original language	English
Pages (from-to)	253-258
Number of pages	6
Journal	Sensors and Actuators B: Chemical
Volume	137
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2008.11.050
Publication status	Published - 28 Mar 2009
Externally published	Yes

Keywords

Electrochemical impedance spectroscopy
Papain
Peptides
SPR
Sensors

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10.1016/j.snb.2008.11.050

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title = "Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain: AC voltammetry, impedance spectroscopy and surface plasmon resonance studies",

abstract = "The interaction of papain with peptide immobilized on the Au surface altered the interfacial electron transfer resistant, charge transfer resistance (RCT), by preventing the redox species approaching the electrode was characterized by electrochemical impedance spectroscopy (EIS), AC voltammetry, cyclic voltammetry and surface plasmon resonance techniques (SPR). From cyclic voltammetry a potential shift of 25-30 mV was observed after the addition of papain, with decrease in current and the impedance results showed that RCT increased with increasing concentration of papain indicating the interaction of papain with the immobilized film. This change in kinetics was also observed in SPR. The interaction was further confirmed by reflection-absorption infrared spectroscopy (RAIR).",

keywords = "Electrochemical impedance spectroscopy, Papain, Peptides, SPR, Sensors",

author = "Tharamani, \{Chikka N.\} and Mahmoud, \{Khaled A.\} and Vasanthakumar, \{Ganga Ramu\} and Kraatz, \{Heinz Bernard\}",

year = "2009",

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doi = "10.1016/j.snb.2008.11.050",

language = "English",

volume = "137",

pages = "253--258",

journal = "Sensors and Actuators B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

number = "1",

}

Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain: AC voltammetry, impedance spectroscopy and surface plasmon resonance studies. / Tharamani, Chikka N.; Mahmoud, Khaled A.; Vasanthakumar, Ganga Ramu et al.
In: Sensors and Actuators B: Chemical, Vol. 137, No. 1, 28.03.2009, p. 253-258.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain

T2 - AC voltammetry, impedance spectroscopy and surface plasmon resonance studies

AU - Tharamani, Chikka N.

AU - Mahmoud, Khaled A.

AU - Vasanthakumar, Ganga Ramu

AU - Kraatz, Heinz Bernard

PY - 2009/3/28

Y1 - 2009/3/28

N2 - The interaction of papain with peptide immobilized on the Au surface altered the interfacial electron transfer resistant, charge transfer resistance (RCT), by preventing the redox species approaching the electrode was characterized by electrochemical impedance spectroscopy (EIS), AC voltammetry, cyclic voltammetry and surface plasmon resonance techniques (SPR). From cyclic voltammetry a potential shift of 25-30 mV was observed after the addition of papain, with decrease in current and the impedance results showed that RCT increased with increasing concentration of papain indicating the interaction of papain with the immobilized film. This change in kinetics was also observed in SPR. The interaction was further confirmed by reflection-absorption infrared spectroscopy (RAIR).

AB - The interaction of papain with peptide immobilized on the Au surface altered the interfacial electron transfer resistant, charge transfer resistance (RCT), by preventing the redox species approaching the electrode was characterized by electrochemical impedance spectroscopy (EIS), AC voltammetry, cyclic voltammetry and surface plasmon resonance techniques (SPR). From cyclic voltammetry a potential shift of 25-30 mV was observed after the addition of papain, with decrease in current and the impedance results showed that RCT increased with increasing concentration of papain indicating the interaction of papain with the immobilized film. This change in kinetics was also observed in SPR. The interaction was further confirmed by reflection-absorption infrared spectroscopy (RAIR).

KW - Electrochemical impedance spectroscopy

KW - Papain

KW - Peptides

KW - SPR

KW - Sensors

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DO - 10.1016/j.snb.2008.11.050

M3 - Article

AN - SCOPUS:60549092246

SN - 0925-4005

VL - 137

SP - 253

EP - 258

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1

ER -

Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain: AC voltammetry, impedance spectroscopy and surface plasmon resonance studies

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Keywords

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