Stable-isotope labeling with amino acids in nematodes

Mark Larance; Aymeric P. Bailly; Ehsan Pourkarimi; Ronald T. Hay; Grant Buchanan; Sarah Coulthurst; Dimitris P. Xirodimas; Anton Gartner; Angus I. Lamond

doi:10.1038/nmeth.1679

Stable-isotope labeling with amino acids in nematodes

Mark Larance, Aymeric P. Bailly, Ehsan Pourkarimi, Ronald T. Hay, Grant Buchanan, Sarah Coulthurst, Dimitris P. Xirodimas, Anton Gartner, Angus I. Lamond^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

99 Citations (Scopus)

Abstract

We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine-and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

Original language	English
Pages (from-to)	849-851
Number of pages	3
Journal	Nature Methods
Volume	8
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.1679
Publication status	Published - Oct 2011
Externally published	Yes

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10.1038/nmeth.1679

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title = "Stable-isotope labeling with amino acids in nematodes",

abstract = "We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine-and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.",

author = "Mark Larance and Bailly, \{Aymeric P.\} and Ehsan Pourkarimi and Hay, \{Ronald T.\} and Grant Buchanan and Sarah Coulthurst and Xirodimas, \{Dimitris P.\} and Anton Gartner and Lamond, \{Angus I.\}",

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AU - Larance, Mark

AU - Bailly, Aymeric P.

AU - Pourkarimi, Ehsan

AU - Hay, Ronald T.

AU - Buchanan, Grant

AU - Coulthurst, Sarah

AU - Xirodimas, Dimitris P.

AU - Gartner, Anton

AU - Lamond, Angus I.

PY - 2011/10

Y1 - 2011/10

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AB - We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine-and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.

UR - https://www.scopus.com/pages/publications/80053363595

U2 - 10.1038/nmeth.1679

DO - 10.1038/nmeth.1679

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VL - 8

SP - 849

EP - 851

JO - Nature Methods

JF - Nature Methods

IS - 10

ER -

Stable-isotope labeling with amino acids in nematodes

Abstract

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