Abstract
Intercellular cognate receptor-ligand pairs are major players in cellular signal transduction. The fact that both the receptor and the ligand are present on the membrane in these juxtacrine signaling interactions presents distinct experimental difficulties in their study. One experimental platform that has proven particularly useful is the hybrid live cell-supported lipid bilayer system, wherein a live cell is allowed to interact with a synthetic supported membrane displaying ligands of interest. A synthetic membrane enables control over identity, density, mobility, and spatial patterning of the displayed ligands. This chapter provides insights gained from the reconstitution of the immunological synapse formed by T-cells, junction formed by ephrinA1-EphA2 receptor tyrosine kinase in breast cancer cells, and adhesion formed by E-cadherin in epithelial cells on synthetic supported lipid bilayers.
| Original language | English |
|---|---|
| Title of host publication | Physics of Biological Membranes |
| Publisher | Springer International Publishing |
| Pages | 537-560 |
| Number of pages | 24 |
| ISBN (Electronic) | 9783030006303 |
| ISBN (Print) | 9783030006280 |
| DOIs | |
| Publication status | Published - 30 Dec 2018 |
| Externally published | Yes |
Keywords
- Cadherin
- Cell signaling
- Eph receptor
- Ephrin
- Immunological synapse
- Mechanical signaling
- Peptide-major histocompatibility complex
- Receptor-ligand clustering
- Supported lipid bilayer
- T-cell receptor
- α-Catenin