SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction

Jennifer Pasquier; Nadine Abu-Kaoud; Houari Abdesselem; Aisha Madani; Jessica Hoarau-Véchot; Hamda Al Thawadi; Fabien Vidal; Bettina Couderc; Gilles Favre; Arash Rafii

doi:10.1186/s12885-015-1556-7

SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction

Jennifer Pasquier, Nadine Abu-Kaoud, Houari Abdesselem, Aisha Madani, Jessica Hoarau-Véchot, Hamda Al Thawadi, Fabien Vidal, Bettina Couderc, Gilles Favre, Arash Rafii^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.

Original language	English
Article number	569
Journal	BMC Cancer
Volume	15
Issue number	1
DOIs	https://doi.org/10.1186/s12885-015-1556-7
Publication status	Published - 1 Aug 2015
Externally published	Yes

Keywords

Breast cancer
Metastasis
SDF-1alpha
Stromal cells
Tumor microenvironment

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10.1186/s12885-015-1556-7

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@article{12d9e671069f4cb580bb26f010f8190c,

title = "SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction",

abstract = "Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.",

keywords = "Breast cancer, Metastasis, SDF-1alpha, Stromal cells, Tumor microenvironment",

author = "Jennifer Pasquier and Nadine Abu-Kaoud and Houari Abdesselem and Aisha Madani and Jessica Hoarau-V{\'e}chot and Thawadi, \{Hamda Al\} and Fabien Vidal and Bettina Couderc and Gilles Favre and Arash Rafii",

note = "Publisher Copyright: {\textcopyright} 2015 Pasquier et al.",

year = "2015",

month = aug,

day = "1",

doi = "10.1186/s12885-015-1556-7",

language = "English",

volume = "15",

journal = "BMC Cancer",

issn = "1471-2407",

publisher = "BioMed Central Ltd",

number = "1",

}

TY - JOUR

T1 - SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction

AU - Pasquier, Jennifer

AU - Abu-Kaoud, Nadine

AU - Abdesselem, Houari

AU - Madani, Aisha

AU - Hoarau-Véchot, Jessica

AU - Thawadi, Hamda Al

AU - Vidal, Fabien

AU - Couderc, Bettina

AU - Favre, Gilles

AU - Rafii, Arash

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.

AB - Background: The interaction of SDF-1alpha with its receptor CXCR4 plays a role in the occurrence of distant metastasis in many solid tumors. This interaction increases migration from primary sites as well as homing at distant sites. Methods: Here we investigated how SDF-1aα could modulate both migration and adhesion of cancer cells through the modulation of RhoGTPases. Results: We show that different concentrations of SDF-1aα modulate the balance of adhesion and migration in cancer cells. Increased migration was obtained at 50 and 100ng/ml of SDF-1aα however migration was reduced at 200ng/ml. The adhesion between breast cancer cells and BMHC was significantly increased by SDF-1aα treatment at 200ng/ml and reduced using a blocking monoclonal antibody against CXCR4. We showed that at low SDF-1aα concentration, RhoA was activated and overexpressed, while at high concentration Rac1 was promoting SDF-1aα mediating-cell adhesion. Conclusion: We conclude that SDF-1aα concentration modulates migration and adhesion of brebreast cancer cells, by controlling expression and activation of RhoGTPases.

KW - Breast cancer

KW - Metastasis

KW - SDF-1alpha

KW - Stromal cells

KW - Tumor microenvironment

UR - https://www.scopus.com/pages/publications/84938055758

U2 - 10.1186/s12885-015-1556-7

DO - 10.1186/s12885-015-1556-7

M3 - Article

C2 - 26231656

AN - SCOPUS:84938055758

SN - 1471-2407

VL - 15

JO - BMC Cancer

JF - BMC Cancer

IS - 1

M1 - 569

ER -

SDF-1alpha concentration dependent modulation of RhoA and Rac1 modifies breast cancer and stromal cells interaction

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