Abstract
Background/Aim: Altered expression of runt-related transcription factor 1 (RUNX1T1) has been observed in several human cancer types; however, the exact role for RUNX1T1 in colorectal cancer (CRC) remains unclear. Materials and Methods: The GSE21510 CRC and our previously published datasets were utilized in this study. Gene-expression profiling was conducted using the Agilent microarray platform, while data normalization and bioinformatics were conducted using GeneSpring software. AlamarBlue assay was used to assess cell viability in vitro. Results: The expression of RUNX1T1 was severely down-regulated in primary CRC and cell lines. Lentiviral-mediated re-expression of RUNX1T1 inhibited CRC cell growth, and global gene-expression analysis revealed the cell cycle, DNA replication, and DNA damage as the pathways most affected by RUNX1T1. Forced expression of RUNX1T1 induced a significant reduction in cellular proliferation and sensitized CRC cells to 5-flurouracil. Conclusion: Our data revealed a novel role for RUNX1T1 as a tumor-suppressor gene in CRC through modulation of multiple cellular pathways.
| Original language | English |
|---|---|
| Pages (from-to) | 5257-5263 |
| Number of pages | 7 |
| Journal | Anticancer Research |
| Volume | 36 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2016 |
| Externally published | Yes |
Keywords
- Chemoresistance
- Colorectal cancer
- Gene expression
- RUNX1T1