Protocol for generating embedding-free brain organoids enriched with oligodendrocytes

Bahaa Al-mhanawi; Marta Boira Marti; Sean D. Morrison; Pallavi Gupta; Maath Alani; Peter G. Noakes; Ernst J. Wolvetang; Mohammed R. Shaker

doi:10.1016/j.xpro.2023.102725

Protocol for generating embedding-free brain organoids enriched with oligodendrocytes

Bahaa Al-mhanawi
, Marta Boira Marti
, Sean D. Morrison
, Pallavi Gupta
, Maath Alani
, Peter G. Noakes
, Ernst J. Wolvetang^*
, Mohammed R. Shaker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

In response to the scarcity of advanced in vitro models dedicated to human CNS white matter research, we present a protocol to generate neuroectoderm-derived embedding-free human brain organoids enriched with oligodendrocytes. We describe steps for neuroectoderm differentiation, development of neural spheroids, and their transferal to Matrigel. We then detail procedures for the development, maturation, and application of oligodendrocyte-enriched brain organoids. The presence of myelin-producing cells makes these organoids useful for studying human white matter diseases, such as leukodystrophy.

Original language	English
Article number	102725
Journal	STAR Protocols
Volume	4
Issue number	4
Early online date	Nov 2023
DOIs	https://doi.org/10.1016/j.xpro.2023.102725
Publication status	Published - 15 Dec 2023
Externally published	Yes

Keywords

Model

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10.1016/j.xpro.2023.102725

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title = "Protocol for generating embedding-free brain organoids enriched with oligodendrocytes",

abstract = "In response to the scarcity of advanced in vitro models dedicated to human CNS white matter research, we present a protocol to generate neuroectoderm-derived embedding-free human brain organoids enriched with oligodendrocytes. We describe steps for neuroectoderm differentiation, development of neural spheroids, and their transferal to Matrigel. We then detail procedures for the development, maturation, and application of oligodendrocyte-enriched brain organoids. The presence of myelin-producing cells makes these organoids useful for studying human white matter diseases, such as leukodystrophy.",

keywords = "Model",

author = "Bahaa Al-mhanawi and Marti, \{Marta Boira\} and Morrison, \{Sean D.\} and Pallavi Gupta and Maath Alani and Noakes, \{Peter G.\} and Wolvetang, \{Ernst J.\} and Shaker, \{Mohammed R.\}",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2023",

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day = "15",

doi = "10.1016/j.xpro.2023.102725",

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TY - JOUR

T1 - Protocol for generating embedding-free brain organoids enriched with oligodendrocytes

AU - Al-mhanawi, Bahaa

AU - Marti, Marta Boira

AU - Morrison, Sean D.

AU - Gupta, Pallavi

AU - Alani, Maath

AU - Noakes, Peter G.

AU - Wolvetang, Ernst J.

AU - Shaker, Mohammed R.

PY - 2023/12/15

Y1 - 2023/12/15

N2 - In response to the scarcity of advanced in vitro models dedicated to human CNS white matter research, we present a protocol to generate neuroectoderm-derived embedding-free human brain organoids enriched with oligodendrocytes. We describe steps for neuroectoderm differentiation, development of neural spheroids, and their transferal to Matrigel. We then detail procedures for the development, maturation, and application of oligodendrocyte-enriched brain organoids. The presence of myelin-producing cells makes these organoids useful for studying human white matter diseases, such as leukodystrophy.

AB - In response to the scarcity of advanced in vitro models dedicated to human CNS white matter research, we present a protocol to generate neuroectoderm-derived embedding-free human brain organoids enriched with oligodendrocytes. We describe steps for neuroectoderm differentiation, development of neural spheroids, and their transferal to Matrigel. We then detail procedures for the development, maturation, and application of oligodendrocyte-enriched brain organoids. The presence of myelin-producing cells makes these organoids useful for studying human white matter diseases, such as leukodystrophy.

KW - Model

UR - https://www.scopus.com/pages/publications/85176797042

U2 - 10.1016/j.xpro.2023.102725

DO - 10.1016/j.xpro.2023.102725

M3 - Article

C2 - 37976154

AN - SCOPUS:85176797042

SN - 2666-1667

VL - 4

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 102725

ER -

Protocol for generating embedding-free brain organoids enriched with oligodendrocytes

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Keywords

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