Probing cII and himA action at the integrase promoter p1 of bacteriophage lambda

Michael Benedik; Desmond Mascarenhas; Allan Campbell

doi:10.1016/0378-1119(82)90020-8

Probing cII and himA action at the integrase promoter p₁ of bacteriophage lambda

Michael Benedik^*, Desmond Mascarenhas, Allan Campbell

^*Corresponding author for this work

Stanford University

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Plasmids were constructed to supply cII-coded protein for activation of the phage promoter p₁. Using a fusion which expresses lacZ from p₁, we can accurately follow activation of p₁ without having to assay int activity in vivo. A large excess of cII protein compared to a normal lytic infection stimulates lacZ expression about 10-fold over the basal level. The int-c226 constitutive allele of p₁ is not further activated by cII even though its level of lacZ expression is less than the maximal cII-activated expression from wild type p₁. A himA-deleted strain also shows activation, demonstrating that there is no absolute requirement for the himA gene product for ell-stimulated transcription at p_I.

Original language	English
Pages (from-to)	303-311
Number of pages	9
Journal	Gene
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/0378-1119(82)90020-8
Publication status	Published - Oct 1982
Externally published	Yes

Keywords

E. coli phage
Gene cloning
deletions
hydroxylamine mutagenesis of plasmids
recombinant DNA
trp-lac fusions

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10.1016/0378-1119(82)90020-8

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title = "Probing cII and himA action at the integrase promoter p1 of bacteriophage lambda",

abstract = "Plasmids were constructed to supply cII-coded protein for activation of the phage promoter p1. Using a fusion which expresses lacZ from p1, we can accurately follow activation of p1 without having to assay int activity in vivo. A large excess of cII protein compared to a normal lytic infection stimulates lacZ expression about 10-fold over the basal level. The int-c226 constitutive allele of p1 is not further activated by cII even though its level of lacZ expression is less than the maximal cII-activated expression from wild type p1. A himA-deleted strain also shows activation, demonstrating that there is no absolute requirement for the himA gene product for ell-stimulated transcription at pI.",

keywords = "E. coli phage, Gene cloning, deletions, hydroxylamine mutagenesis of plasmids, recombinant DNA, trp-lac fusions",

author = "Michael Benedik and Desmond Mascarenhas and Allan Campbell",

year = "1982",

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doi = "10.1016/0378-1119(82)90020-8",

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journal = "Gene",

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T1 - Probing cII and himA action at the integrase promoter p1 of bacteriophage lambda

AU - Benedik, Michael

AU - Mascarenhas, Desmond

AU - Campbell, Allan

PY - 1982/10

Y1 - 1982/10

N2 - Plasmids were constructed to supply cII-coded protein for activation of the phage promoter p1. Using a fusion which expresses lacZ from p1, we can accurately follow activation of p1 without having to assay int activity in vivo. A large excess of cII protein compared to a normal lytic infection stimulates lacZ expression about 10-fold over the basal level. The int-c226 constitutive allele of p1 is not further activated by cII even though its level of lacZ expression is less than the maximal cII-activated expression from wild type p1. A himA-deleted strain also shows activation, demonstrating that there is no absolute requirement for the himA gene product for ell-stimulated transcription at pI.

AB - Plasmids were constructed to supply cII-coded protein for activation of the phage promoter p1. Using a fusion which expresses lacZ from p1, we can accurately follow activation of p1 without having to assay int activity in vivo. A large excess of cII protein compared to a normal lytic infection stimulates lacZ expression about 10-fold over the basal level. The int-c226 constitutive allele of p1 is not further activated by cII even though its level of lacZ expression is less than the maximal cII-activated expression from wild type p1. A himA-deleted strain also shows activation, demonstrating that there is no absolute requirement for the himA gene product for ell-stimulated transcription at pI.

KW - E. coli phage

KW - Gene cloning

KW - deletions

KW - hydroxylamine mutagenesis of plasmids

KW - recombinant DNA

KW - trp-lac fusions

UR - https://www.scopus.com/pages/publications/0020406332

U2 - 10.1016/0378-1119(82)90020-8

DO - 10.1016/0378-1119(82)90020-8

M3 - Article

C2 - 6295882

AN - SCOPUS:0020406332

SN - 0378-1119

VL - 19

SP - 303

EP - 311

JO - Gene

JF - Gene

IS - 3

ER -

Probing cII and himA action at the integrase promoter p₁ of bacteriophage lambda

Abstract

Keywords

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