Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements

Caitlin Howell; Hicham Hamoudi; Stefan Heissler; Patrick Koelsch; Michael Zharnikov

doi:10.1016/j.cplett.2011.07.096

Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements

Caitlin Howell, Hicham Hamoudi, Stefan Heissler, Patrick Koelsch^*, Michael Zharnikov

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5 mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2 mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems.

Original language	English
Pages (from-to)	267-270
Number of pages	4
Journal	Chemical Physics Letters
Volume	513
Issue number	4-6
DOIs	https://doi.org/10.1016/j.cplett.2011.07.096
Publication status	Published - 15 Sept 2011
Externally published	Yes

Access to Document

10.1016/j.cplett.2011.07.096

Cite this

@article{e966aa7d52f8479e87167f48d3f44adc,

title = "Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements",

abstract = "Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5 mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2 mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems.",

author = "Caitlin Howell and Hicham Hamoudi and Stefan Heissler and Patrick Koelsch and Michael Zharnikov",

year = "2011",

month = sep,

day = "15",

doi = "10.1016/j.cplett.2011.07.096",

language = "English",

volume = "513",

pages = "267--270",

journal = "Chemical Physics Letters",

issn = "0009-2614",

publisher = "Elsevier B.V.",

number = "4-6",

}

TY - JOUR

T1 - Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements

AU - Howell, Caitlin

AU - Hamoudi, Hicham

AU - Heissler, Stefan

AU - Koelsch, Patrick

AU - Zharnikov, Michael

PY - 2011/9/15

Y1 - 2011/9/15

N2 - Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5 mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2 mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems.

AB - Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5 mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2 mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems.

UR - https://www.scopus.com/pages/publications/80052458994

U2 - 10.1016/j.cplett.2011.07.096

DO - 10.1016/j.cplett.2011.07.096

M3 - Article

AN - SCOPUS:80052458994

SN - 0009-2614

VL - 513

SP - 267

EP - 270

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 4-6

ER -

Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements

Abstract

Access to Document

Other files and links

Fingerprint

Cite this