Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

Masaru Okuda; Henning F. Horn; Pheruza Tarapore; Yukari Tokuyama; A. George Smulian; Pui Kwong Chan; Erik S. Knudsen; Irene A. Hofmann; Jean D. Snyder; Kevin E. Bove; Kenji Fukasawa

doi:10.1016/S0092-8674(00)00093-3

Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

Masaru Okuda
, Henning F. Horn
, Pheruza Tarapore
, Yukari Tokuyama
, A. George Smulian
, Pui Kwong Chan
, Erik S. Knudsen
, Irene A. Hofmann
, Jean D. Snyder
, Kevin E. Bove
, Kenji Fukasawa^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

593 Citations (Scopus)

Abstract

In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.

Original language	English
Pages (from-to)	127-140
Number of pages	14
Journal	Cell
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/S0092-8674(00)00093-3
Publication status	Published - 29 Sept 2000
Externally published	Yes

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10.1016/S0092-8674(00)00093-3

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@article{8760891a25b743f1b03e9564febd1142,

title = "Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication",

abstract = "In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.",

author = "Masaru Okuda and Horn, \{Henning F.\} and Pheruza Tarapore and Yukari Tokuyama and Smulian, \{A. George\} and Chan, \{Pui Kwong\} and Knudsen, \{Erik S.\} and Hofmann, \{Irene A.\} and Snyder, \{Jean D.\} and Bove, \{Kevin E.\} and Kenji Fukasawa",

year = "2000",

month = sep,

day = "29",

doi = "10.1016/S0092-8674(00)00093-3",

language = "English",

volume = "103",

pages = "127--140",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "1",

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TY - JOUR

T1 - Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

AU - Okuda, Masaru

AU - Horn, Henning F.

AU - Tarapore, Pheruza

AU - Tokuyama, Yukari

AU - Smulian, A. George

AU - Chan, Pui Kwong

AU - Knudsen, Erik S.

AU - Hofmann, Irene A.

AU - Snyder, Jean D.

AU - Bove, Kevin E.

AU - Fukasawa, Kenji

PY - 2000/9/29

Y1 - 2000/9/29

N2 - In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.

AB - In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.

UR - https://www.scopus.com/pages/publications/0034730321

U2 - 10.1016/S0092-8674(00)00093-3

DO - 10.1016/S0092-8674(00)00093-3

M3 - Article

C2 - 11051553

AN - SCOPUS:0034730321

SN - 0092-8674

VL - 103

SP - 127

EP - 140

JO - Cell

JF - Cell

IS - 1

ER -

Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

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