Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism

M. N. Filimonova; V. P. Gubskaya; I. A. Nuretdinov; M. J. Benedik; L. M. Bogomolnaya; M. A. Andreeva; I. B. Leshchinskaya

Isoforms of Serratia marcescens nuclease. The role of Mg²⁺ in the hydrolysis mechanism

M. N. Filimonova^*, V. P. Gubskaya, I. A. Nuretdinov, M. J. Benedik, L. M. Bogomolnaya, M. A. Andreeva, I. B. Leshchinskaya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg²⁺ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclcasc has been summarized on the basis of experimental data. Mg²⁺ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate.

Original language	English
Pages (from-to)	983-988
Number of pages	6
Journal	Biochemistry (Moscow)
Volume	62
Issue number	9
Publication status	Published - 1997
Externally published	Yes

Keywords

CD
Isoforms
Mg
Mg-DNA
Na DNA
Nuclease
Serratia marcescens

Cite this

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title = "Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism",

abstract = "Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclcasc has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate.",

keywords = "CD, Isoforms, Mg, Mg-DNA, Na DNA, Nuclease, Serratia marcescens",

author = "Filimonova, \{M. N.\} and Gubskaya, \{V. P.\} and Nuretdinov, \{I. A.\} and Benedik, \{M. J.\} and Bogomolnaya, \{L. M.\} and Andreeva, \{M. A.\} and Leshchinskaya, \{I. B.\}",

year = "1997",

language = "English",

volume = "62",

pages = "983--988",

journal = "Biochemistry (Moscow)",

issn = "0006-2979",

publisher = "Pleiades Publishing",

number = "9",

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TY - JOUR

T1 - Isoforms of Serratia marcescens nuclease. The role of Mg2+ in the hydrolysis mechanism

AU - Filimonova, M. N.

AU - Gubskaya, V. P.

AU - Nuretdinov, I. A.

AU - Benedik, M. J.

AU - Bogomolnaya, L. M.

AU - Andreeva, M. A.

AU - Leshchinskaya, I. B.

PY - 1997

Y1 - 1997

N2 - Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclcasc has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate.

AB - Structural and functional differences between isoforms Sm1 and Sm2, a lack of influence of free Mg2+ on the isoform structures, formation of DNA-magnesium complex serving with great probability as a real substrate for the nuclcasc has been summarized on the basis of experimental data. Mg2+ forming a complex with phosphate groups of DNA are supposed to further increase the electrophilicity of the phosphorus atoms besides causing a conformational change of the substrate.

KW - CD

KW - Isoforms

KW - Mg

KW - Mg-DNA

KW - Na DNA

KW - Nuclease

KW - Serratia marcescens

UR - https://www.scopus.com/pages/publications/0031217890

M3 - Article

C2 - 9457762

AN - SCOPUS:0031217890

SN - 0006-2979

VL - 62

SP - 983

EP - 988

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 9

ER -

Isoforms of Serratia marcescens nuclease. The role of Mg²⁺ in the hydrolysis mechanism

Abstract

Keywords

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