Ectopic B7-H4-Ig expression attenuates concanavalin A-induced hepatic injury

Jun Fa Xu; Huan Xiao; Guo Yan Hu; Shu Hua Zheng; Wei Liu; Chun Lei Yuan; Heng Yang; Jing Lü; Fang Zheng; Cong Yi Wang; Fei Li Gong

doi:10.1016/j.clim.2010.02.022

Ectopic B7-H4-Ig expression attenuates concanavalin A-induced hepatic injury

Jun Fa Xu^*
, Huan Xiao
, Guo Yan Hu
, Shu Hua Zheng
, Wei Liu
, Chun Lei Yuan
, Heng Yang
, Jing Lü
, Fang Zheng
, Cong Yi Wang
, Fei Li Gong

^*Corresponding author for this work

Guangdong Medical College
Huazhong University of Science and Technology
Department of Pathology
Augusta University

Research output: Contribution to journal › Article › peer-review

Abstract

Previous studies demonstrate that both membrane B7-H4 and B7-H4-Ig fusion protein could inhibit T-cell responses. In the present study, we explored the potential effect of B7-H4-Ig on liver injury in a hepatitis mouse model induced by concanavalin A (ConA). A B7-H4-Ig construct was introduced into animals by the hydrodynamic gene delivery approach. It was found that ectopic expression of B7-H4-Ig could inhibit ConA-induced elevation of serum levels of ALT and AST, suppress liver necrosis and even mortality of mice. Furthermore, we observed that pretreatment of B7-H4-Ig dramatically decreased serum levels and the expression of mRNA for IL-2, IFN-γ and IL-4, but increased IL-10 in ConA-treated mice. Our results suggest that B7-H4-Ig may protect animals from liver injury induced by ConA, which could be associated with reduced serum levels for IL-2, IFN-γ and IL-4 as well as enhanced IL-10 production.

Original language	English
Pages (from-to)	30-41
Number of pages	12
Journal	Clinical Immunology
Volume	136
Issue number	1
DOIs	https://doi.org/10.1016/j.clim.2010.02.022
Publication status	Published - Jul 2010
Externally published	Yes

Keywords

B7-H4
concanavalin A
hepatic injury
hydrodynamics- based gene delivery
IFN-γ
IL-10
IL-2
IL-4

Access to Document

10.1016/j.clim.2010.02.022

Cite this

@article{67e5996093c04bf1a825d179408fdf7a,

title = "Ectopic B7-H4-Ig expression attenuates concanavalin A-induced hepatic injury",

abstract = "Previous studies demonstrate that both membrane B7-H4 and B7-H4-Ig fusion protein could inhibit T-cell responses. In the present study, we explored the potential effect of B7-H4-Ig on liver injury in a hepatitis mouse model induced by concanavalin A (ConA). A B7-H4-Ig construct was introduced into animals by the hydrodynamic gene delivery approach. It was found that ectopic expression of B7-H4-Ig could inhibit ConA-induced elevation of serum levels of ALT and AST, suppress liver necrosis and even mortality of mice. Furthermore, we observed that pretreatment of B7-H4-Ig dramatically decreased serum levels and the expression of mRNA for IL-2, IFN-γ and IL-4, but increased IL-10 in ConA-treated mice. Our results suggest that B7-H4-Ig may protect animals from liver injury induced by ConA, which could be associated with reduced serum levels for IL-2, IFN-γ and IL-4 as well as enhanced IL-10 production.",

keywords = "B7-H4, concanavalin A, hepatic injury, hydrodynamics- based gene delivery, IFN-γ, IL-10, IL-2, IL-4",

author = "Xu, \{Jun Fa\} and Huan Xiao and Hu, \{Guo Yan\} and Zheng, \{Shu Hua\} and Wei Liu and Yuan, \{Chun Lei\} and Heng Yang and Jing L{\"u} and Fang Zheng and Wang, \{Cong Yi\} and Gong, \{Fei Li\}",

year = "2010",

month = jul,

doi = "10.1016/j.clim.2010.02.022",

language = "English",

volume = "136",

pages = "30--41",

journal = "Clinical Immunology",

issn = "1521-6616",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Ectopic B7-H4-Ig expression attenuates concanavalin A-induced hepatic injury

AU - Xu, Jun Fa

AU - Xiao, Huan

AU - Hu, Guo Yan

AU - Zheng, Shu Hua

AU - Liu, Wei

AU - Yuan, Chun Lei

AU - Yang, Heng

AU - Lü, Jing

AU - Zheng, Fang

AU - Wang, Cong Yi

AU - Gong, Fei Li

PY - 2010/7

Y1 - 2010/7

N2 - Previous studies demonstrate that both membrane B7-H4 and B7-H4-Ig fusion protein could inhibit T-cell responses. In the present study, we explored the potential effect of B7-H4-Ig on liver injury in a hepatitis mouse model induced by concanavalin A (ConA). A B7-H4-Ig construct was introduced into animals by the hydrodynamic gene delivery approach. It was found that ectopic expression of B7-H4-Ig could inhibit ConA-induced elevation of serum levels of ALT and AST, suppress liver necrosis and even mortality of mice. Furthermore, we observed that pretreatment of B7-H4-Ig dramatically decreased serum levels and the expression of mRNA for IL-2, IFN-γ and IL-4, but increased IL-10 in ConA-treated mice. Our results suggest that B7-H4-Ig may protect animals from liver injury induced by ConA, which could be associated with reduced serum levels for IL-2, IFN-γ and IL-4 as well as enhanced IL-10 production.

AB - Previous studies demonstrate that both membrane B7-H4 and B7-H4-Ig fusion protein could inhibit T-cell responses. In the present study, we explored the potential effect of B7-H4-Ig on liver injury in a hepatitis mouse model induced by concanavalin A (ConA). A B7-H4-Ig construct was introduced into animals by the hydrodynamic gene delivery approach. It was found that ectopic expression of B7-H4-Ig could inhibit ConA-induced elevation of serum levels of ALT and AST, suppress liver necrosis and even mortality of mice. Furthermore, we observed that pretreatment of B7-H4-Ig dramatically decreased serum levels and the expression of mRNA for IL-2, IFN-γ and IL-4, but increased IL-10 in ConA-treated mice. Our results suggest that B7-H4-Ig may protect animals from liver injury induced by ConA, which could be associated with reduced serum levels for IL-2, IFN-γ and IL-4 as well as enhanced IL-10 production.

KW - B7-H4

KW - concanavalin A

KW - hepatic injury

KW - hydrodynamics- based gene delivery

KW - IFN-γ

KW - IL-10

KW - IL-2

KW - IL-4

UR - https://www.scopus.com/pages/publications/77953028044

U2 - 10.1016/j.clim.2010.02.022

DO - 10.1016/j.clim.2010.02.022

M3 - Article

C2 - 20346734

AN - SCOPUS:77953028044

SN - 1521-6616

VL - 136

SP - 30

EP - 41

JO - Clinical Immunology

JF - Clinical Immunology

IS - 1

ER -

Ectopic B7-H4-Ig expression attenuates concanavalin A-induced hepatic injury

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this