Disulfide bonds are required for Serratia marcescens nuclease activity

Timothy K. Ball; Yousin Suh; Michael J. Benedik

doi:10.1093/nar/20.19.4971

Disulfide bonds are required for Serratia marcescens nuclease activity

Timothy K. Ball
, Yousin Suh
, Michael J. Benedik^*

^*Corresponding author for this work

University of Houston

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

Original language	English
Pages (from-to)	4971-4974
Number of pages	4
Journal	Nucleic Acids Research
Volume	20
Issue number	19
DOIs	https://doi.org/10.1093/nar/20.19.4971
Publication status	Published - 11 Oct 1992
Externally published	Yes

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title = "Disulfide bonds are required for Serratia marcescens nuclease activity",

abstract = "The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.",

author = "Ball, \{Timothy K.\} and Yousin Suh and Benedik, \{Michael J.\}",

year = "1992",

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doi = "10.1093/nar/20.19.4971",

language = "English",

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T1 - Disulfide bonds are required for Serratia marcescens nuclease activity

AU - Ball, Timothy K.

AU - Suh, Yousin

AU - Benedik, Michael J.

PY - 1992/10/11

Y1 - 1992/10/11

N2 - The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

AB - The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

UR - https://www.scopus.com/pages/publications/0026675734

U2 - 10.1093/nar/20.19.4971

DO - 10.1093/nar/20.19.4971

M3 - Article

C2 - 1329033

AN - SCOPUS:0026675734

SN - 0305-1048

VL - 20

SP - 4971

EP - 4974

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 19

ER -

Disulfide bonds are required for Serratia marcescens nuclease activity

Abstract

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