Cyanide bioremediation: the potential of engineered nitrilases

Jason M. Park; B. Trevor Sewell; Michael J. Benedik

doi:10.1007/s00253-017-8204-x

Cyanide bioremediation: the potential of engineered nitrilases

Jason M. Park, B. Trevor Sewell, Michael J. Benedik^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

62 Citations (Scopus)

Abstract

The cyanide-degrading nitrilases are of notable interest for their potential to remediate cyanide contaminated waste streams, especially as generated in the gold mining, pharmaceutical, and electroplating industries. This review provides a brief overview of cyanide remediation in general but with a particular focus on the cyanide-degrading nitrilases. These are of special interest as the hydrolysis reaction does not require secondary substrates or cofactors, making these enzymes particularly good candidates for industrial remediation processes. The genetic approaches that have been used to date for engineering improved enzymes are described; however, recent structural insights provide a promising new approach.

Original language	English
Pages (from-to)	3029-3042
Number of pages	14
Journal	Applied Microbiology and Biotechnology
Volume	101
Issue number	8
DOIs	https://doi.org/10.1007/s00253-017-8204-x
Publication status	Published - 1 Apr 2017
Externally published	Yes

Keywords

Bioremediation
Cyanide
Cyanide dihydratase
Nitrilase
Protein engineering
Protein stability

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10.1007/s00253-017-8204-x

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title = "Cyanide bioremediation: the potential of engineered nitrilases",

abstract = "The cyanide-degrading nitrilases are of notable interest for their potential to remediate cyanide contaminated waste streams, especially as generated in the gold mining, pharmaceutical, and electroplating industries. This review provides a brief overview of cyanide remediation in general but with a particular focus on the cyanide-degrading nitrilases. These are of special interest as the hydrolysis reaction does not require secondary substrates or cofactors, making these enzymes particularly good candidates for industrial remediation processes. The genetic approaches that have been used to date for engineering improved enzymes are described; however, recent structural insights provide a promising new approach.",

keywords = "Bioremediation, Cyanide, Cyanide dihydratase, Nitrilase, Protein engineering, Protein stability",

author = "Park, \{Jason M.\} and \{Trevor Sewell\}, B. and Benedik, \{Michael J.\}",

note = "Publisher Copyright: {\textcopyright} 2017, Springer-Verlag Berlin Heidelberg.",

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doi = "10.1007/s00253-017-8204-x",

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T1 - Cyanide bioremediation

T2 - the potential of engineered nitrilases

AU - Park, Jason M.

AU - Trevor Sewell, B.

AU - Benedik, Michael J.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The cyanide-degrading nitrilases are of notable interest for their potential to remediate cyanide contaminated waste streams, especially as generated in the gold mining, pharmaceutical, and electroplating industries. This review provides a brief overview of cyanide remediation in general but with a particular focus on the cyanide-degrading nitrilases. These are of special interest as the hydrolysis reaction does not require secondary substrates or cofactors, making these enzymes particularly good candidates for industrial remediation processes. The genetic approaches that have been used to date for engineering improved enzymes are described; however, recent structural insights provide a promising new approach.

AB - The cyanide-degrading nitrilases are of notable interest for their potential to remediate cyanide contaminated waste streams, especially as generated in the gold mining, pharmaceutical, and electroplating industries. This review provides a brief overview of cyanide remediation in general but with a particular focus on the cyanide-degrading nitrilases. These are of special interest as the hydrolysis reaction does not require secondary substrates or cofactors, making these enzymes particularly good candidates for industrial remediation processes. The genetic approaches that have been used to date for engineering improved enzymes are described; however, recent structural insights provide a promising new approach.

KW - Bioremediation

KW - Cyanide

KW - Cyanide dihydratase

KW - Nitrilase

KW - Protein engineering

KW - Protein stability

UR - https://www.scopus.com/pages/publications/85014568479

U2 - 10.1007/s00253-017-8204-x

DO - 10.1007/s00253-017-8204-x

M3 - Review article

C2 - 28265723

AN - SCOPUS:85014568479

SN - 0175-7598

VL - 101

SP - 3029

EP - 3042

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 8

ER -

Cyanide bioremediation: the potential of engineered nitrilases

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Keywords

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