Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

Raúl Teruel; Irene Martínez-Martínez; José A. Guerrero; Rocío González-Conejero; María E. De La Morena-Barrio; Salam Salloum-Asfar; Ana B. Arroyo; Sonia Águila; Nuria García-Barberá; Antonia Miñano; Vicente Vicente; Javier Corral; Constantino Martínez

doi:10.1186/1423-0127-20-29

Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

Raúl Teruel
, Irene Martínez-Martínez
, José A. Guerrero
, Rocío González-Conejero
, María E. De La Morena-Barrio
, Salam Salloum-Asfar
, Ana B. Arroyo
, Sonia Águila
, Nuria García-Barberá
, Antonia Miñano
, Vicente Vicente
, Javier Corral
, Constantino Martínez^*

^*Corresponding author for this work

University of Murcia

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Background: Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control. Conclusions: These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.

Original language	English
Article number	29
Journal	Journal of Biomedical Science
Volume	20
Issue number	1
DOIs	https://doi.org/10.1186/1423-0127-20-29
Publication status	Published - 2013
Externally published	Yes

Keywords

Antithrombin
Microarray
Post-natal development
Post-translational modifications
Sialytransferases
miRNAs

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10.1186/1423-0127-20-29

Cite this

Teruel, R., Martínez-Martínez, I., Guerrero, J. A., González-Conejero, R., De La Morena-Barrio, M. E., Salloum-Asfar, S., Arroyo, A. B., Águila, S., García-Barberá, N., Miñano, A., Vicente, V., Corral, J., & Martínez, C. (2013). Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a. Journal of Biomedical Science, 20(1), Article 29. https://doi.org/10.1186/1423-0127-20-29

@article{fa70282212e34e91a52846124c5950bf,

title = "Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a",

abstract = "Background: Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85\% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control. Conclusions: These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.",

keywords = "Antithrombin, Microarray, Post-natal development, Post-translational modifications, Sialytransferases, miRNAs",

author = "Ra{\'u}l Teruel and Irene Mart{\'i}nez-Mart{\'i}nez and Guerrero, \{Jos{\'e} A.\} and Roc{\'i}o Gonz{\'a}lez-Conejero and \{De La Morena-Barrio\}, \{Mar{\'i}a E.\} and Salam Salloum-Asfar and Arroyo, \{Ana B.\} and Sonia {\'A}guila and Nuria Garc{\'i}a-Barber{\'a} and Antonia Mi{\~n}ano and Vicente Vicente and Javier Corral and Constantino Mart{\'i}nez",

year = "2013",

doi = "10.1186/1423-0127-20-29",

language = "English",

volume = "20",

journal = "Journal of Biomedical Science",

issn = "1021-7770",

publisher = "BioMed Central Ltd",

number = "1",

}

Teruel, R, Martínez-Martínez, I, Guerrero, JA, González-Conejero, R, De La Morena-Barrio, ME, Salloum-Asfar, S, Arroyo, AB, Águila, S, García-Barberá, N, Miñano, A, Vicente, V, Corral, J & Martínez, C 2013, 'Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a', Journal of Biomedical Science, vol. 20, no. 1, 29. https://doi.org/10.1186/1423-0127-20-29

TY - JOUR

T1 - Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

AU - Teruel, Raúl

AU - Martínez-Martínez, Irene

AU - Guerrero, José A.

AU - González-Conejero, Rocío

AU - De La Morena-Barrio, María E.

AU - Salloum-Asfar, Salam

AU - Arroyo, Ana B.

AU - Águila, Sonia

AU - García-Barberá, Nuria

AU - Miñano, Antonia

AU - Vicente, Vicente

AU - Corral, Javier

AU - Martínez, Constantino

PY - 2013

Y1 - 2013

N2 - Background: Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control. Conclusions: These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.

AB - Background: Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control. Conclusions: These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.

KW - Antithrombin

KW - Microarray

KW - Post-natal development

KW - Post-translational modifications

KW - Sialytransferases

KW - miRNAs

UR - https://www.scopus.com/pages/publications/84878809389

U2 - 10.1186/1423-0127-20-29

DO - 10.1186/1423-0127-20-29

M3 - Article

C2 - 23678987

AN - SCOPUS:84878809389

SN - 1021-7770

VL - 20

JO - Journal of Biomedical Science

JF - Journal of Biomedical Science

IS - 1

M1 - 29

ER -

Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

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Keywords

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