@inproceedings{52fea77cb7a547feabd473377022491f,
title = "Biomedical applications of two-foci cross-correlation technique in massively parallel fluorescence correlation spectroscopy",
abstract = "We report the use of two-foci cross-correlation in a massively parallel fluorescence correlation spectroscopy (mpFCS) setup to map the spatial distribution and motion of fluorescent molecules inside living cells. The mpFCS experimental setup enables the simultaneous measurement of raw photon counts from a matrix of confocal observation volume elements (OVEs) generated in parallel. To determine the average number of fluorescent molecules in each OVE, their diffusion times, and the two-dimensional velocity components in the imaging plane, we compute autocorrelation functions (ACFs) and two-foci cross-correlation functions (tfCCFs) after each data acquisition. We demonstrate the power of this approach by measuring, in live HEK 293 cells expressing the Glucocorticoid Receptor tagged at the Nterminus with Enhanced Green Fluorescent Protein (eGFP-GR), the directionality of nucleocytoplasmic transport.",
keywords = "Fluorescence Correlation Spectroscopy, Nucleocytoplasmic Transport of Glucocorticoid Receptor",
author = "Nikolic, \{Stanko N.\} and Sho Oasa and Krmpot, \{Aleksandar J.\} and Lars Terenius and Beli{\'c}, \{Milivoj R.\} and Vladana Vukojevi{\'c}",
note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; 23rd Dynamics and Fluctuations in Biomedical Photonics ; Conference date: 17-01-2026 Through 19-01-2026",
year = "2026",
month = mar,
day = "5",
doi = "10.1117/12.3090147",
language = "English",
isbn = "978-1-5106-9613-6",
volume = "13850",
series = "Progress In Biomedical Optics And Imaging",
publisher = "SPIE",
editor = "VV Tuchin and MJ Leahy and RK Wang",
booktitle = "Dynamics and Fluctuations in Biomedical Photonics XXIII",
address = "United States",
}