A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

Khaled A. Mahmoud; John H.T. Luong

doi:10.1080/00032711003653924

A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

Khaled A. Mahmoud
, John H.T. Luong

National Research Council of Canada

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.

Original language	English
Pages (from-to)	1680-1687
Number of pages	8
Journal	Analytical Letters
Volume	43
Issue number	10-11
DOIs	https://doi.org/10.1080/00032711003653924
Publication status	Published - Jul 2010
Externally published	Yes

Keywords

Electrochemistry
Ferrocene
Gold nanoparticle
Hiv-1
Pepstatin
Protease
Single-walled carbon nanotube

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10.1080/00032711003653924

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title = "A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode",

abstract = "A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.",

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AU - Mahmoud, Khaled A.

AU - Luong, John H.T.

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N2 - A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.

AB - A rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.

KW - Electrochemistry

KW - Ferrocene

KW - Gold nanoparticle

KW - Hiv-1

KW - Pepstatin

KW - Protease

KW - Single-walled carbon nanotube

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DO - 10.1080/00032711003653924

M3 - Article

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VL - 43

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JO - Analytical Letters

JF - Analytical Letters

IS - 10-11

ER -

A sensitive electrochemical assay for early detection of HIv-1 protease using ferrocenepeptide conjugate/AU nanoparticle/single walled carbon nanotube modified electrode

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Keywords

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